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Meals & nutrition

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Maverick Diaz
Maverick Diaz

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A total of 128 museum specimens and 556 museum specimens were included in the vwf locus sequencing. In addition, 136 field specimens sequenced in previous studies were included in the vwf locus sequencing. All of these individuals had a unique haplotype. The phylogenetic tree for the vwf locus ( Additional file 1 : Figure S7) was constructed using maximum likelihood methods. The resulting phylogenetic tree (Additional file 1 : Figure S7) was consistent with the phylogenetic tree of [ 55 ] that was built from the same dataset with maximum likelihood methods. The phylogenetic tree was also consistent with the phylogenetic tree of Ezaki et al [ 46 ] that used a Bayesian implementation. The number of individuals in the phylogenetic tree shown in Additional file 1 : Figure S7 is 107 which includes all 11 COBB clades.]




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After the alignments were completed using the software MAFFT [ 56 ] and edited manually using the software MEGA 5.0 [ 57 ], the final ML tree was generated by Modeltest 2.4 (BIC) and Phyml version 3.0.1010. The Maximum Likelihood tree (Additional file 1 : Figure S8) generated by the best fitting model (GTR+I+G) and the Bayesian tree (Additional file 1 : Figure S9) were highly congruent with the Modeltest tree. Analyses of the phylogenetic tree for each COBB clade were not performed due to the limited number of samples of clades E and F. Clade G was excluded from further analyses due to very low sample size. In addition, the branch lengths of the nodes in the Modeltest tree (Additional file 1 : Figure S7) were highly heterogeneous (i.e. some nodes support relationships with confidence levels of 0.8) and short. Therefore, the branch length of the clade G was very short compared to other nodes in the tree, and the resolution of the tree from this clade is unreliable. To test the reliability of the phylogeny that was obtained with maximum likelihood, especially clade G and E, we selected 47 individuals from the Modeltest tree to generate the phylogenetic tree using maximum likelihood in the software MEGA 5.0. Both the ML tree ( Additional file 1 : Figure S10) and the Bayesian tree ( Additional file 1 : Figure S11) were highly consistent with the Modeltest tree. Support values for each node in the ML and Bayesian phylogenetic trees are shown in Additional file 1 : Table S8. The phylogeny of Eulemur spp. based on the three loci used in this study is consistent with the hypothesis that the members of the BLC are monophyletic taxa. The high bootstrap support values (>95) for the remaining nodes in the tree further support the monophyly of the BLC ( Additional file 1 : Figure S12).


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